Cast:[]
- Ash Ketchum as Flik
- Rei Hino/Sailor Mars as Princess Atta
- Lilo as Dot
- Miss Miller as The Queen
- Pikachu as Aphie
- Max and Enrique as Dot's 2 Boyfriends
- Ranma Saotome as Mr. Soil
- Moana as Dr. Flora
- Spike Taylor as Thorny
- Professor Oak as Cornelius
- Dr. Z as Hopper
- Seth as Molt
- HIM as Thumper
- Zander and Ed as Axel and Loco
- Doc as P.T. Flea
- Major Glory as Francis
- Valhallen as Slim
- The Infragible Krunk as Heimlich
- Buzz Lightyear (Buzz Lightyear of Star Command) as Dim
- Usagi Tsukino/Sailor Moon as Gypsy
- Mamoru Chiba/Tuxedo Mask as Manny
- Zoe Drake as Rosie
- Fred Flintstone and Barney Rubble as Tuck and Roll
- Phineas and Ferb as Fly Brothers
- Dr. Doofenshmirtz as Thud
- Fiona as the Bird
- Coming Soon to YouTube
Other Casts:[]
- Ant who shouts "I'm lost!" - Tulio (The Road to El Dorado)
- Ant that gets tired - Rai (Tai Chi Chasers)
- Grasshoppers - Various Villains Humans
- Grasshopper next to Mort, Axel and Loco - Clayton (Tarzan)
- 2 Grasshoppers that Dot overhears - James and Butch (Pokemon; 1997)
- Blueberry Troop Kids - Various Children
- Baby Maggots - Baby Roshan (Ice Age), and Baby Tarzan (Tarzan)
- Harry and Bug friend - Fflewddur Fflamm and Taran (The Black Cauldron)
- Cricket with "Kid Pulled My Wings Off" sign - Maui (Moana)
- Mime Bug - Dee Dee as a Mime (Dexter's Laboratory: The Laughing)
- Cockroach Waitress - Lum (Urusei Yatsura)
- Mosquito Waitress - Andrina (The Little Mermaid)
- Slick - Genie (Aladdin)
- Mosquito who drinks 'Bloody Mary' - Ron Stoppable (Kim Possible)
- Baby Birds - Farkle, Fergus, and Felicia (Shrek)
- Woody (in Outtakes) - Max Taylor (Dinosaur King)